Reporter
Part:BBa_E0050:Design
Designed by: Austin Che and Reshma Shetty Group: iGEM07_Example (2007-07-23)
GFP.lacZalpha fusion
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644
Design Notes
I have included the complete GFP sequence. The boundaries of the lacZ alpha fragment are the first 60 codons of lacZ. This is what is defined as the alpha peptide in "The lac operon" by Benno Muller-Hill (see page 76).
Source
The GFP sequence comes from (BBa_E0040) and the N-terminal alpha fragment of lacZ comes directly from lacZ.
References
- Benno Muller-Hill. The Lac Operon. Walter de Gruyter. isbn:3-11-014830-7. [Muller-Hill-1996]